ChemLeader Biomedical Co., Limited

Product Name: Y-27632 dihydrochloride; Y-27632 2HCl

CAS No.: 129830-38-2; 146986-50-7

Chemical Name: trans-4-[(1R)-1-Aminoethyl]-N-4-pyridinylcyclohexanecarboxamide dihydrochloride; 4-[(1R)-1-Aminoethyl]-N-pyridin-4-ylcyclohexane-1-carboxamide dihydrochloride; (R)-(+)-trans-4-(1-Aminoethyl)-N-(4-pyridyl)cyclohexanecarboxamide dihydrochloride; (1R,4r)-4-((R)-1-aminoethyl)-N-(pyridin-4-yl)cyclohexanecarboxamide

Molecular Formula: C₁₄H₂₁N₃O.2HCl

Molecular Weight:  320.26

Appearance:White solid
Purity(HPLC): >99%

Biological Activity: Selective inhibitor of the Rho-associated protein kinase p160ROCK. K_ivalues are 0.14, 26, 25 and > 250 M for p160ROCK, PKC, cAMP-dependent protein kinase and myosin light-chain kinase respectively. Also inhibits the protein kinase C-related protein kinase, PRK2 (IC₅₀= 600 nM). Smooth muscle relaxant and orally active in vivo. Increases survival rate of human embryonic stem (hES) cells undergoing cryopreservation.  Y-27632 is a cell-permeable, highly potent and selective inhibitor of Rho-associated, coiled-coil containing protein kinase (ROCK). Y-27632 inhibits both ROCKI (K_i= 220 nM) and ROCKII (K_i = 300 nM) by competing with ATP for binding to the catalytic site. Y-27632 is a biochemical tool used in the study of the rho-associated protein kinase (ROCK) signaling pathways. Y-27632 selectively inhibits p160ROCK.
Y-27632 is highly potent, cell-permeable, selective ROCK (Rho-associated coiled coil forming protein serine/threonine kinase) inhibitor. K_i= 140 nM for p160^ROCK. Y-27632 also inhibits ROCK-II with equal potency. The inhibition is competitive with respect to ATP.