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Compound | lithium acetate | ||||
---|---|---|---|---|---|
CAS No. | 546-89-4 | Catalog No. | XMZX-W13692 | Brand | |
Purity | 99% | Packing | 25kg/DRUMS | Grade | |
Lead Time | 3Day (s) | Origin | Loading Port |
Boiling Point | 117.1ºC at 760 mmHg |
---|---|
Stability | Stable. Incompatible with strong oxidizing agents. |
Storage Condition | Store in a tightly closed container. Store in a cool, dry, well-ventilated area away from incompatible substances. |
Appearance & Physical State | solid |
Water Solubility | 40.8 g/100 mL (20 ºC) |
Flash Point | 40ºC |
Melting Point | 280-285ºC |
Chemical Properties solid
Uses Lithium acetate is used in the laboratory as buffer for gel electrophoresis of DNA and RNA. It has a lower electrical conductivity and can be run at higher speeds than can gels made from TAE buffer (5-30V/cm as compared to 5 - 10 V / cm). Lithium boric acid or sodium boric acid are usually preferable to lithium acetate or TAE when analyzing smaller fragments of DNA (less than 500 bp) due to the higher resolution of borate-based buffers in this size range as compared to acetate buffers.
Lithium acetate is also used to permeabilize the cell wall of yeast for use in DNA transformation. It is believed that the beneficial effect of LiAc is caused by its chaotropic effect denaturing both DNA, RNA and proteins.
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